Facts About flow cytometers Revealed
Facts About flow cytometers Revealed
Blog Article
Information-enriched characterization of cell populations. Morphological details from pictures can incorporate for the richness of flow cytometry knowledge. By way of example, the figure displays an normally regular apoptosis assay making use of Annexin V and PI, adding mobile imaging to characterize cells in Every single inhabitants to reveal morphologically distinctive options. These insights couldn't happen to be received from multiplex staining alone.
As samples are acquired on the Attune CytPix Flow Cytometer, the large-speed brightfield camera captures and stores photos of detected gatherings at a charge around six,000 pictures for every next, with regards to the flow amount and graphic measurement. For increased versatility, Attune Cytometric Computer software lets you adjust the graphic seize frequency as desired and choose a specific gate for image capture, if essential.
(B) Uncooked 264.7 macrophages tend to be annotated inaccurately with regard to particle rely because of their complexity and noncircular borders. The number of singlets obtainable for Evaluation boosts once the person trains and employs a more correct model.
The award-successful Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing by using a large-pace brightfield digicam for simultaneous large throughput flow cytometry and superior resolution brightfield imaging.
*Pricing is indicative only and is particularly subject matter to vary. Please contact your Agilent representative with any thoughts. The data established forth herein will not be a suggestion or possibly a motivation, the two of that happen to be issue to credit history acceptance, conditions and terms, and availability with Agilent. Agilent Financial Solutions is obtainable in picked countries.
Additionally, it is illuminating to determine the quantity of doublets slip by traditional singlet gates, and terrific to verify the status of unpredicted double-good situations in my Investigation."
Pair your Attune flow cytometer with the autosampler for improved workflow performance, and include robotic integration for max throughput.
Regular picture good quality even at higher throughput Acoustic focusing in addition to a substantial-velocity digital camera Mix to impression these T cells regularly at lower or higher flow costs. Conveniently adjust focus and camera options to meet experimental prerequisites.
Early signs of this contamination experienced in the beginning been dismissed as particles. Backgating to find new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also allows you to use morphological features to find out appealing subpopulations that would not be apparent from flow cytometry knowledge by yourself.
Customers can check out celebration pictures in serious-time to verify sample excellent, optimize protocols, and record the morphology of each mobile inhabitants recognized for Examination. Imaging capture also Gains from acoustic concentrating, which helps you to place the cells to ensure that a sharp, centered graphic is acquired.
AI-driven automatic image Examination with the Attune Cytometric Program translates event attributes into distinctive morphology parameters which might be coupled with standard fluorescence and scatter parameters to expose hugely reproducible inhabitants improvements and unique subsets.
Software note: Automated graphic Examination decreases consumer-to-person variability in flow cytometry gating techniques
Leverage the power of Superior engineering and innovation to accelerate your discoveries. Rely on from the Attune Flow Cytometer spouse and children to provide the trustworthiness, velocity, and precision that established your get the job done apart.
No clean/no lyse leukocyte Examination with imaging. Cells have been obtained from 24-hour-outdated blood by dilution in one mM EDTA ( Pro UX/UI allows customers to teach AI types for expanded applications. (A) The “Train Design” characteristic gives annotation tools and real-time model schooling precision.
Gating on these prolonged parameters means that you can quickly and correctly detect populations of fascination and make sure gating tactic with little or no guide review. The Attune software presents entry to state-of-the-art equipment Mastering Assessment tools inside of an expertly developed person interface, rendering it exceptionally convenient to use.
As the cell line experienced Earlier been analyzed about the Attune CytPix Flow Cytometer, the researchers went again to the images and had been in a position more info to document the microbial an infection at the very least 5 times earlier.
Agilent Benefit Promise assures you at least ten years of instrument use in the date you purchase. Usually, We are going to credit rating you While using the residual value of the procedure towards an upgraded product.
What appeared to be a homogeneous populace is definitely a lot more numerous—an insight that ought to be regarded as when interpreting outcomes.